optimization of buffalo (bubalus bubalis) embryonic stem cell culture system
نویسندگان
چکیده
0
منابع مشابه
Optimization of Buffalo (Bubalus bubalis) Embryonic Stem Cell Culture System
OBJECTIVE In order to retain an undifferentiated pluripotent state, embryonic stem (ES) cells have to be cultured on feeder cell layers. However, use of feeder layers limits stem cell research, since experimental data may result from a combined ES cell and feeder cell response to various stimuli. MATERIALS AND METHODS In this experimental study, a buffalo ES cell line was established from in ...
متن کاملWNT3A signalling pathway in buffalo (Bubalus bubalis) embryonic stem cells.
The aim of this study was to investigate the transcriptional profile and role of WNT3A signalling in maintaining buffalo embryonic stem (ES) cells in a pluripotent state and in the induction of their differentiation. ES cells were derived from embryos produced by in vitro fertilisation (iESC), parthenogenesis (pESC) and hand-made cloning (cESC). The expression of WNT3A, its receptors and interm...
متن کاملEmbryonic Development of Heart in Indian Buffalo (Bubalus bubalis)
The present study was conducted on 35 buffalo foetuses from 0.9 cm CVRL (32 days) to 99.5 cm CVRL (298 days) to observe the morphogenesis and histogenesis of heart. The study revealed that, in 0.9 cm CVRL buffalo foetus, heart was unseptated and tubular which was clearly divided into common atrial chamber dorsally, primitive ventricles ventrally, primitive outflow tract with bulbous cordis regi...
متن کاملSpermatogonial Stem Cells (SSCs) in Buffalo (Bubalus bubalis) Testis
BACKGROUND Water buffalo is an economically important livestock species and about half of its total world population exists in India. Development of stem cell technology in buffalo can find application in targeted genetic modification of this species. Testis has emerged as a source of pluripotent stem cells in mice and human; however, not much information is available in buffalo. OBJECTIVES A...
متن کاملStorage of buffalo (Bubalus bubalis) semen.
Characteristics of buffalo semen, diluents used for liquid storage, aspects involved in freezing and thawing of semen are reviewed, and fertility results after artificial insemination (AI) with frozen-thawed semen are given.
متن کاملCongenital Malformations in River Buffalo (Bubalus bubalis)
The world buffalo population is about 168 million, and it is still growing, in India, China, Brazil, and Italy. In these countries, buffalo genetic breeding programs have been performed for many decades. The occurrence of congenital malformations has caused a slowing of the genetic progress and economic loss for the breeders, due to the death of animals, or damage to their reproductive ability ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
cell journalجلد ۱۷، شماره ۲، صفحات ۲۶۴-۲۷۲
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023